| NDN

Necdin is a protein that is encoded by the NDN gene.This intronless gene is located in the Prader-Willi syndrome (PWS) deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mice suggest that the protein encoded by this gene may suppress growth in postmitotic neurons.Necdin is used to stimulate growth regulation and DNA-dependent transcription regulation.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.44	1.87	1.29	0.83	0.58	0.38	0.27	0.08
Corrected OD	2.36	1.79	1.21	0.75	0.5	0.3	0.19	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human NDN spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	91-105	102

 

| Linearity

Samples were spiked with high concentrations of Human NDN and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)
1 : 2	Range (%)	85-96
	Average (%)	93
1 : 4	Range (%)	87-108
	Average (%)	97